antivimentin (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology antivimentin
Antivimentin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antivimentin/Vimentin+Antibody/pm41928272-82-15-16
Average 96 stars, based on 3039 article reviews

antivimentin - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: miR-7213-5p-mediated suppression of CCL19 in fibroblast cells may attenuate lupus nephritis.
Article Snippet: .. Primary antibody incubation was carried out overnight at 4 °C using antiCCL19 (sc-74233, Santa Cruz Biotechnology) and antiVimentin (sc-66002, Santa Cruz Biotechnology) antibodies. .. Detection was performed using the TSAPLus fluorescence double-labeling three-color staining kit (G1226-50T, Servicebio).

Article Title: The deubiquitinase BRCC3 increases the stability of ZEB1 and promotes the proliferation and metastasis of triple-negative breast cancer cells.
Article Snippet: Cell lysates were collected, centrifuged and subsequently measured using a BCA kit (Sigma). .. Fifty micrograms of protein sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to PVDF membranes (Millipore, Billerica, USA), blocked with 5% skim milk for 1 h and incubated overnight with primary antibodies as follows: anti-ZEB1 (ab203829, 1:500; Abcam, Cambridge, UK), anti-STAMBP (sc-398480, 1:1000; Santa Cruz Biotech, Santa Cruz, USA), anti-BAP1 (ab245391, 1:2000; Abcam), anti-UCHL1 (sc-271639, 1:1000; Santa Cruz Biotech), antiUSP9X (ab19879, 1:1000; Abcam), anti-BRCC3 (ab115172, 1:2000; Abcam), anti-E-cadherin (sc-8426, 1:1000; Santa Cruz Biotech), antivimentin (sc-6260, 1:1000; Santa Cruz Biotech), and anti-β-actin (ab227387, 1:5000; Abcam). .. Themembrane was washed three times with TBST and incubated with an HRP-conjugated secondary antibody (1:3000; Cell Signaling Technology, Beverly, USA) for 30 min. After extensive wash, protein bands were visualized using ECL plus regent (Beyotime, Shanghai, China).

Article Title: Zika virus E protein modulates functions of human brain microvascular endothelial cells and astrocytes: implications on blood-brain barrier properties.
Article Snippet: .. The blots were incubated with respective primary antibodies: antiZIKV-E protein (1:2000, GeneTex, Cat# GTX133314), anti-ZO-1 (1:2,000, Invitrogen, Cat# 61-7300), anti-VE-Cadherin (1:2,000, Abcam, Cat# ab33168), anti-Occludin (1:500, Invitrogen, Cat# OC-3F10), anti-GFAP (1:60,000, Dako, USA, Cat# Z0334), antiVimentin (1:10,000, Santa Cruz Biotechnology, USA, Cat# sc6260), and anti-β-actin (1:40,000, Sigma Cat# A3854), overnight at 4◦C. .. The blots were washed with 1X TBST thrice for 5 min and then incubated with HRP- conjugated secondary antibodies (1:4,000, Vector Labs, Burlingame, CA, USA) for 1–2 h at RT.

Nucleic Acid Electrophoresis:

Immunoprecipitation:

Article Title: Guanxin V alleviates ventricular remodeling by promoting transforming growth factor-beta 1-mediated proteasomal degradation of Vimentin.
Article Snippet: Lysate proteins from HEK 293T cells were immunoprecipitated with magnetic beads (MedChemExpress) and tested by immunoblotting using anti-HA (Cell Signaling Technology, MA) and anti-MYC (Cell Signaling Technology) antibodies. .. To assess their endogenous interaction, TGF-b1 and Vimentin proteins from H9c2(2-1) cardiomyocytes and heart tissues were immunoprecipitated with antiTGF-b1 (Santa Cruz Biotechnology, TX) and antiVimentin (Santa Cruz Biotechnology) antibodies and detected by immunoblotting. ..

Western Blot:

Article Title: AKAP2 is required for assembly of cytoskeletal signaling complexes that promote growth and metastasis of triple-negative breast cancer.
Article Snippet: .. Primary antibodies used for immunoblotting include: anti-AKAP2 (1:1000, Novus Biological, NB-100-61604); anti-e-cadherin (1:1000, BD Transduction, 610181); anti-ZEB1 (1:1000, CST, 70512T); anti-β-actin (1:1000, CST, 4970S); antivimentin (1:1000, Santa Cruz Biotechnology, sc-6260); anti-Streptavidin-HRP (1:10000, abcam 7403); anti-FAK (1:1000, Proteintech, 66258-1-Ig); anti-paxillin (1:1000, Proteintech, 10029-1-Ig); anti-MMP1 (1:2000, Proteintech, 10371-2-AP); or MMP2 (1:1000, 10373-2-AP). ..

Article Title: IL11 signaling mediates piR-2158 suppression of cell stemness and angiogenesis in breast cancer.
Article Snippet: .. The following primary antibodies were used for western blot (1:1,000 dilution): anti-Slug (sc-166476, Santa Cruz), antiVimentin (sc-32322, Santa Cruz), anti-Fibronectin (sc-8422, Santa Cruz), anti-ZEB1 (sc-515797, Santa Cruz), anti-KLF4 (ARG-55811, Arigo Inc.), antiNANOG (4903S, Cell Signaling Technology), antiSOX2 (3579, Cell Signaling Technology), anti-OCT4 (2750S, Cell Signaling Technology), anti-IL-11 (55169-1-AP, Proteintech), anti-STAT3 (4904, Cell Signaling Technology), anti-Phospho-STAT3 (Tyr705) (9145T, Cell Signaling Technology), anti-GAPDH (sc-47724, Santa Cruz) and anti-β-actin (sc-47778, Santa Cruz). .. HRP-linked anti-rabbit IgG (7074S, Cell Signaling Technology) and HRP-linked anti-mouse IgG (7076S, Cell Signaling Technology) were used as secondary antibodies (1:5,000 dilution).

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